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Development of an HPLC assay for the analysis of tetrafluoroputrescine—A putrescine analog

✍ Scribed by Amale A. Hawi; Helen Yip; Teresa S. Sullivan; George A. Digenis

Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
472 KB
Volume
172
Category
Article
ISSN
0003-2697

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✦ Synopsis

A simple reverse-phase liquid chromatographic system with ultraviolet detection at 254 nm for tetrafluoroputrescine (TFP), a putrescine analog, is described. The assay involves precolumn derivatization of TFP with dansyl chloride at pH 6.8-7.5 at 60°C. followed by separation from putrescine (PUT) and quantitation.

The derivatization procedure was adapted for the simultaneous analysis of TFP and PUT in whole blood components.

Also, preliminary studies on protein binding of TFP are reported.

cl 1988 Academic PW. IIIC.

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