Development of a topographically organized auditory network in slice culture is calcium dependent
✍ Scribed by Lohmann, Christian ;Ilic, Vesna ;Friauf, Eckhard
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 910 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0022-3034
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✦ Synopsis
Inhibitory and excitatory connecblockers (MgCl 2 and nifedipine) to the high-KCl metions of remarkably precise topographic order are dium reduced organotypicity drastically, indicating characteristic features of the mammalian auditory that a depolarization-induced increase of intracellular system, particularly within the superior olivary comcalcium is indispensable. Furthermore, the temporal plex (SOC). Little is known about the requirements course of the expression of the calcium-binding profor the correct development of these specific connectein parvalbumin in culture under high KCl mimics tions. Previous in vivo experiments have demonstrated that in vivo, demonstrating developmental processes a high expression of calcium-binding proteins in this during incubation. The need for calcium influx into system during development, pointing to the need for neurons of this auditory network in vitro (which is precise calcium regulation. Here, we have employed not seen in other slice culture systems) strengthens an organotypic slice culture from the above neuronal the hypothesis that an optimal calcium concentration network and analyzed the requirements for the mainis exceptionally important in auditory neurons. The tenance and development of this system in vitro. When effect of KCl in the slice cultures may substitute for slices from neonatal rats were incubated in standard input activity regulating intracellular calcium in audiculture medium for up to 7 days, we found no organotory neurons in vivo. ᭧ 1998 John Wiley & Sons, Inc. J typic features. Only if 25 mM KCl was added to the Neurobiol 34: 97-112, 1998 culture medium, the cytoarchitecture of the nuclei, the