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Development of a Time-Resolved Fluorometric Method for Observing Hybridization in Living Cells Using Fluorescence Resonance Energy Transfer

✍ Scribed by Tsuji, Akihiko; Sato, Yoshihiro; Hirano, Masahiko; Suga, Takayuki; Koshimoto, Hiroyuki; Taguchi, Takeshi; Ohsuka, Shinji

Book ID
118514060
Publisher
Biophysical Society
Year
2001
Tongue
English
Weight
420 KB
Volume
81
Category
Article
ISSN
0006-3495

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An assay named Cell TR-FRET based on time-resolved fluorescence resonance energy transfer, here utilized for detection of receptor proteins on intact cells, is described. In this assay, intact membrane-biotinylated Sf9 cells expressing human interleukin-2Ralpha due to infection with a recombinant ba