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Development of a Time Domain Fluorimeter for Fluorescent Lifetime Multiplexing Analysis

✍ Scribed by Salthouse, C.D.; Weissleder, R.; Mahmood, U.


Book ID
117906822
Publisher
Institute of Electrical and Electronics Engineers
Year
2008
Tongue
English
Weight
790 KB
Volume
2
Category
Article
ISSN
1932-4545

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## Abstract Frequency‐domain fluorescence lifetime imaging microscopy (FLIM) has become a commonly used technique to measure lifetimes in biological systems. However, lifetime measurements are strongly dependent on numerous experimental parameters. Here, we describe a complete calibration and chara