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Development of a specific radioimmunoassay for vitamin B12and its application in the diagnosis of cobalt deficiency in sheep

✍ Scribed by D. G. Kennedy; F. P. M. O'Harte; W. J. Blanchflower; D. A. Rice

Publisher
Springer Netherlands
Year
1990
Tongue
English
Weight
696 KB
Volume
14
Category
Article
ISSN
0165-7380

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✦ Synopsis

A radioimmunoassay (KU) for vitamin B is described. Antisera were raised in rabbits using a conjugate between S-0-succinyl cyanocoba#min and chicken serum albumin. The antibody is largely specific for the fi (upper) face of the vitamin B molecule. The RfA can detect vitamin B down to concentrations of 37 pmol/L and correlated we&r = 0.980,~ < 0.001) with a commercial +adioassay. The use of this RIA, together with an assay for plasma methylmalonic acid, in the diagnosis of cobalt/vitamin B12 deficiency in sheep is described.

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