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Development of a simple fed-batch process for the high-yield production of recombinant Japanese encephalitis virus protein

✍ Scribed by Nagesh K. Tripathi; Jyoti Shukla; Karttik C. Biswal; P. V. Lakshmana Rao


Publisher
Springer
Year
2010
Tongue
English
Weight
229 KB
Volume
86
Category
Article
ISSN
1432-0614

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A fed-batch cell culture process was developed that has general applicability to all evaluated Sp2/0 (n = 8) and NS0 (n = 1) antibody-producing cell lines. The two key elements of this generic process were a protein-free concentrated feed medium, and a robust, metabolically responsive feeding strate