A high-yielding, generic fed-batch cell
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Paul W. Sauer; John E. Burky; Mark C. Wesson; Heather D. Sternard; Limin Qu
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Article
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2000
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John Wiley and Sons
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English
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A fed-batch cell culture process was developed that has general applicability to all evaluated Sp2/0 (n = 8) and NS0 (n = 1) antibody-producing cell lines. The two key elements of this generic process were a protein-free concentrated feed medium, and a robust, metabolically responsive feeding strate