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Development of a set of expression vectors inHansenula polymorpha

โœ Scribed by Houhui Song; Yong Li; Weihuan Fang; Yunfeng Geng; Xu Wang; Min Wang; Bingsheng Qiu

Book ID
111573359
Publisher
Springer Netherlands
Year
2003
Tongue
English
Weight
201 KB
Volume
25
Category
Article
ISSN
0141-5492

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Effect of soybean oil on the enhanced ex
Effect of soybean oil on the enhanced expression of hirudin gene inHansenula polymorpha
โœ Chul-Ho Kim; Jung-Hoon Sohn; Eui-Sung Choi; Sang-Ki Rhee ๐Ÿ“‚ Article ๐Ÿ“… 1996 ๐Ÿ› Springer Netherlands ๐ŸŒ English โš– 415 KB

A heterologous gene from bloodsucking leech for anticoagulant, hirudin, has been expressed in the methylotrophic yeast Hansenula polymorpha. The addition of 1 %(v/v) soybean oil to the medium as a stabilizer enhanced the expression of the hirudin gene in H. polymorpha with MOX promoter. The product