Development of a radioimmunoassay data analysis pack (RIADAP) in level II basic for microcomputers
✍ Scribed by Gilles Forget; Pierre Sirois
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 499 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0010-4809
No coin nor oath required. For personal study only.
✦ Synopsis
A simple program written in Microsoft BASIC for the analysis of radioimmunoassay data is presented. The program was designed for use by unexperienced operators on a TRS-80 Model III microcomputer. Standard curve data are fitted by linear regression after a logit responseflog, dose transformation. Standard errors of individual observations are computed, and confidence intervals are obtained for a critical t of 0.1. Standard observations are eliminated when they lie outside the confidence interval from the calculated response on the fitted curve, and this is accomplished until no datum can be eliminated or until the coefficient of determination reaches an operator defined value. The program then prints the regression parameters and the retained standard values. Unknown sample data can then be entered, and the values are computed relative to the final regression parameters of the standard curve. The values can be modified by operator defined correction/dilution factors. The mean value of the slope for 28 assays is -0.99, while the predicted theoretical slope with a log, transformation is -1. This program finds its greatest use in research laboratories where a number of dierent RIA are performed and where it is of value to have some control over the computation process.
Endocrinol. Suppl. 142, 163 (1969).