Quantitative real-time PCR for rapid and
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Richard B. Hitchman; Evangelia A. Siaterli; Clare P. Nixon; Linda A. King
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Article
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2007
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John Wiley and Sons
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English
β 120 KB
π 1 views
## Abstract We describe the use of quantitative PCR (QPCR) to titer recombinant baculoviruses. Custom primers and probe were designed to gp64 and used to calculate a standard curve of QPCR derived titers from dilutions of a previously titrated baculovirus stock. Each dilution was titrated by both p