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Development of a quantitative real-time PCR for determination of genotype frequencies for studies in baculovirus population biology

✍ Scribed by Mark P. Zwart; Monique M. van Oers; Jenny S. Cory; Jan W.M. van Lent; Wopke van der Werf; Just M. Vlak


Book ID
113767708
Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
390 KB
Volume
148
Category
Article
ISSN
0166-0934

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## Abstract We describe the use of quantitative PCR (QPCR) to titer recombinant baculoviruses. Custom primers and probe were designed to gp64 and used to calculate a standard curve of QPCR derived titers from dilutions of a previously titrated baculovirus stock. Each dilution was titrated by both p