Development of a quantitative PCR assay for monitoring HCV viraemia levels in patients with chronic hepatitis C
β Scribed by G. Colucci; K. Gutekunst
- Book ID
- 108885629
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 427 KB
- Volume
- 4
- Category
- Article
- ISSN
- 1352-0504
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Quantitative measurement of serum hepatitis C virus (HCV) RNA is important in predicting and monitoring the therapeutic effects of interferon in treating patients with chronic hepatitis C. We compared two commercial available assays, Roche Amplicor HCV Monitor test kits and Chiron branched DNA signa
A modified competitive RT-PCR (mcRT-PCR) to measure HCV RNA in serum and the Amplicor HCV Monitor assay were compared. For mcRT-PCR, the RNA extracted was retrotranscribed and coamplified in one step with a known amount of a DNA internal control (IC). Digoxigenin-labeled amplified products were hybr