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Development of a quantitative competitive polymerase chain reaction for human herpesvirus 8

✍ Scribed by Matthew J. Lock; Paul D. Griffiths; Vincent C. Emery


Book ID
114140329
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
620 KB
Volume
64
Category
Article
ISSN
0166-0934

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Aequorin-based flash-type bioluminescent methods can detect nucleic acid molecules in the attomolar range (10(-18)) enabling improved monitoring of the polymerase chain reaction (PCR) at cycles previously considered too low for product detection. The high sensitivity of bioluminescence (BL) was used