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Development of a PNA Probe for Fluorescence In Situ Hybridization Detection of Prorocentrum donghaiense

✍ Scribed by Chen, Guofu; Zhang, Chunyu; Zhang, Baoyu; Wang, Guangce; Lu, Douding; Xu, Zhong; Yan, Peishen; Waller, Ross Frederick

Book ID
119969573
Publisher
Public Library of Science
Year
2011
Tongue
English
Weight
673 KB
Volume
6
Category
Article
ISSN
1932-6203

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## Abstract Stringency in ribosomal RNA (rRNA)‐targeted fluorescence in situ hybridization (FISH) is typically adjusted with formamide, and the optimum formamide concentration at which the probe can hybridize with the target rRNA, but not with rRNAs with mismatches, is to be found experimentally. T