## Abstract A rapid technique for the detection of hepatitis C virus (HCV) RNA in liver section using noniso‐topic in situ hybridization was described. Only 3 of the 10 patients seropositive for antibody to HCV and HCV RNA had detectable HCV RNA in the hepatocytes (1%, 2%, and 15% of cells positive
Development of a novel nested in situ PCR–ISH method for detection of hepatitis C virus RNA in liver tissue
✍ Scribed by Alhusain J Alzahrani; Pamela J Vallely; Raymond F.T McMahon
- Book ID
- 114139755
- Publisher
- Elsevier Science
- Year
- 2002
- Tongue
- English
- Weight
- 403 KB
- Volume
- 99
- Category
- Article
- ISSN
- 0166-0934
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## Abstract Interpretation of antibody to hepatitis C virus (HCV) in patients with liver disease is difficult due to false‐positive reactivity in some conditions. To evaluate the feasibility of HCV in archival material, HCV was sought in formalin‐fixed, paraffin‐embedded liver biopsy specimens. Nes
The cellular localization of hepatitis C virus (HCV) RNA in liver tissue was studied by nonisotopic in situ hybridization using a digoxigenin-labeled cDNA probe created during a polymerase chain reaction on samples from 16 patients with chronic HCV infection. Hybridization signalswere recognized in