Development of a Novel Helicase Assay Using Electrochemiluminescence

✍ Scribed by Litao Zhang; Geoffrey Schwartz; Mike O'Donnell; Richard K. Harrison

• Book ID: 102567279
• Publisher: Elsevier Science
• Year: 2001
• Tongue: English
• Weight: 147 KB
• Volume: 293
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.2001.5093

✦ Synopsis

DNA helicases are ubiquitous enzymes involved in DNA replication, recombination, and repair. These enzymes use the energy of ATP to unwind duplex DNA. A common approach to measure DNA helicase activity utilizes electrophoresis to visualize product formation in a gel. The present study develops a rapid helicase assay based on electrochemiluminescence. The assay is adaptable to rapid high-throughput screening for chemical inhibitors. The assay is applied to Escherichia coli DnaB. Three other technologies have also been applied to DnaB and are compared to results of the electrochemiluminescence-based assay.