Development of a novel chemiluminescent probe, 4-(5',6'-Dimethoxybenzothiazolyl) phthalhydrazide

✍ Scribed by Junichi Ishida; Maki Takada; Syuuji Hara; Kazumi Sasamoto; Kenyu Kina; Masatoshi Yamaguchi

• Book ID: 102982536
• Publisher: Elsevier Science
• Year: 1995
• Tongue: English
• Weight: 565 KB
• Volume: 309
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(95)00082-b

✦ Synopsis

4-(5',6'-Dimethoxybenzothiazolyl)phthalhydrazide (DBPH) was synthesized as a new, highly sensitive chemiluminescent reagent. The reagent consists of phthalhydrazide and benzothiazole moieties and generates strong chemiluminescence by reaction with hydrogen peroxide in the presence of potassium hexacyanoferrate(II1) or peroxidase. The chemiluminescence intensity of DBPH was about 2U times greater than that of luminol. The detection limit of DBPH was 1.3 X lo-" M [0.65 fmol per well (200 pl), signal to blank ratio = 21. The reaction of DBPH was also applied to the determination of hydrogen peroxide by flow injection. The detection limit is 0.5 pmol for a lOO-/*I injection.