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Development of a novel capillary electrophoresis chemiluminescence system for amino acid analysis

✍ Scribed by Dongguang Yin; Chunjuan Xie; Li Zhang; Binghu Liu; Xiaozhou Zhou; Peng Wang; Minghong Wu

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
218 KB
Volume
23
Category
Article
ISSN
1522-7235

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✦ Synopsis

Abstract

In the present study, a novel peroxyoxalate CE–CL system was developed to achieve high signal stability and sensitivity based on a design of a new interface including a new mixing mode and a new grounding electrode mode. Amino acids fluorescently tagged with dansyl chloride and naphthalene‐2,3‐dicarboxaldehyde(NDA) were used for the study. Experiment results show this new system is quite effective to separate and detect amine acid with high stability and resolute. The detection limits were 1.1Β nmol/L for dansyl‐leucine (Leu) and 2.0Β nmol/L for dansyl‐aspartic acid (Asp). The relative standard deviations of peak height and migration time were in the ranges of 2.3–3.8% and 1.2–1.5%, respectively. Copyright Β© 2008 John Wiley & Sons, Ltd.

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