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Development of a new molecular detection method for Taylorella equigenitalis

✍ Scribed by Akihiro Tazumi; Junichi Hirayama; Kyohei Hayashi; Sandrine Petry; John E. Moore; B. Cherie Millar; Prof. Dr. Motoo Matsuda

Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
327 KB
Volume
51
Category
Article
ISSN
0233-111X

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✦ Synopsis

Abstract

On PCR amplification of the intervening sequences (IVSs) in the central (helix 45) region within 23S rRNA gene sequences with T. equigenitalis (n = 34), as well as T. asinigenitalis (n = 35) and Bordetella (n = 11) isolates by using the primer pair of f‐/r‐23STis2, approximately 0.8 kb of the amplicons were generated, sequenced and analyzed. One IVS of approximately 70 bp in length was identified in all the Taylorella organisms but not Bordetella. PCR amplification was further developed for the convenient and rapid molecular detection of T. equigenitalis organisms with the IVS in the helix 45 region within the 23S rRNA genes as target by using the primer pairs (f‐IVSde/r‐23de). Thus, these results clearly demonstrated that PCR amplification with the primer pair (f‐IVSde/r‐23de) can be reliable in order to differentiate the T. equigenitalis isolates from both the T. asinigenitalis and Bordetella organisms. (Β© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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