Development of a new fluorescence decay measurement system using two-dimensional single-photon counting

✍ Scribed by M. Watanabe; M. Koishi; M. Fujiwara; T. Takeshita; W. Cieslik

• Book ID: 103993940
• Publisher: Elsevier Science
• Year: 1994
• Tongue: English
• Weight: 355 KB
• Volume: 80
• Category: Article
• ISSN: 1010-6030
• DOI: 10.1016/1010-6030(93)01009-q

✦ Synopsis

A new fluorescence decay measurement system has been developed.

The system consists of a spectrograph and a new two-dimensional photon counter. The combination enables measurements to be made of the fluorescence decay as a function of time and wavelength simultaneously.

The time resolution is better than 5 ps with deconvolution processing, and the wavelength resolution is approximately 0.15 nm with 1200 grooves mm-' gratings. The dynamic range is 105. The instrument response function (IRF) of the system is nearly gaussian, and has no tail or "after pulses" which are commonly observed using a photomultiplier in a time-correlated photon counting (TCPC) system. Therefore fast fluorescence decay of several tens of picoseconds can be measured accurately.

In addition, the two-dimensional single-photon counting can be performed without wavelength scanning, so that the wavelengthdependent fluorescence decay can be easily and directly observed with a fast throughput and a high signal-t@ noise ratio. The principle of two-dimensional photon counting is discussed together with characteristics including linearity and statistical behavior.