Development of a micro flow spectrophotometric titration method

✍ Scribed by Fumio Segara; Toshiyuki Kobayashi; Tadayoshi Tajima; Hiroyuki Ijyuin; Isao Yoshida; Daido Ishii; Keihei Ueno

• Book ID: 102624891
• Publisher: Elsevier Science
• Year: 1992
• Tongue: English
• Weight: 321 KB
• Volume: 261
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(92)80232-v

✦ Synopsis

A micro flow spectrophotometnc tltratlon method IS described Commercially avadable flexible fused-sdlca capdlary tubes for gas chromatography were found to have very uniform bore and good flexdnhty, and could be used for constructmg nucroburettes, nucropipettes and nucro-flow cells A solution of less than 120 PLI m a mlcrobeaker (500 @I) was titrated Hrlth EDTA solution from a rmcroburette The solution m the beaker was circulated through the rmcro-flow cell of a spectrophotometrx detector to determme the mchcator end-pomt The dead volume of the cxculatmg system was about 75 ~1 For example, a solution (about 100 ~1) contammg 1 31 pg of zinc, buffer (pH 5 21) and Xylenol Orange mchcator was titrated v&h 0 02 M EDTA solution, the volume of htrant bemg as small as 094 /.LcLI