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Development of a Melanin-Based High-Performance Liquid Chromatography Stationary Phase and Its Use in the Study of Drug-Melanin Binding Interactions

✍ Scribed by H. Ibrahim; A.F. Aubry

Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
476 KB
Volume
229
Category
Article
ISSN
0003-2697

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✦ Synopsis

Drug-melanin interactions were studied using a melanin-based HPLC column. Two approaches were chosen for the preparation of the stationary phases: covalent coupling of synthetic L-dopa melanin and in situ polymerization of L-dopa. Retention of a series of phenothiazines on melanin-based stationary phases was attributed to binding to melanin. Frontal affinity chromatography experiments on one melanin-based column allowed us to calculate the affinity and binding capacity of chlorpromazine and promethazine. A competition was observed between chlorpromazine and haloperidol which was qualitatively consistent with previously described results. Data indicated that the interaction was not a simple competition at one site.

v) 1995 Academic Press, Inc.

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