✦ LIBER ✦

Development of a mass-producible glucose biosensor and flow-injection analysis system suitable for on-line monitoring during fermentations

✍ Scribed by Stephen F. White; Ibtisam E. Tothill; Jeffrey D. Newman; Anthony P.F. Turner

Publisher: Elsevier Science
Year: 1996
Tongue: English
Weight: 610 KB
Volume: 321
Category: Article
ISSN: 0003-2670
DOI: 10.1016/0003-2670(95)00514-5

No coin nor oath required. For personal study only.

✦ Synopsis

Screen printed ampemmetric glucose biosen~ors were constructed and optimised for use in a flow injection analysis (FIA) system. The sensors were fabricated using a catalytic metallised carbon based ink, allowing a working potential of + 350 mV (Ag/AgCI). Overall the sensors displayed a high degree of reliability and robustness. A linear response was obtained over the range 0.1 to 25 mM glucose with a slope of 1.35 + 0.20/tA/mM. The within electrode coefficient of variation at each concentration tested (n = 10) was less than 2% (or 0.1 mM for concentrations below 5 raM). In addition, it was demonstrated that a single sensor can be used in the HA system over a seven day period indicating a high level of operational staldlity, l'hese sensors fulfil the criteria necessary for a mass produced device, which would be suitable for the on-line monitoring of glucose consumption during microbial fermentation.

📜 SIMILAR VOLUMES

Characterization and application of an o

Characterization and application of an on-line flow injection analysis/wall-jet electrode system for glucose monitoring during fermentation

✍ Yu Liang Huang; Titus J. Foellmer; Koon Chye Ang; Soo Beng Khoo; Miranda G.S. Ya 📂 Article 📅 1995 🏛 Elsevier Science 🌐 English ⚖ 879 KB

An on-line system for glucose monitoring during fermentation was developed. The system employed flow-injection analysis coupled with a wall-jet electrode. Glucose was converted enzymatically in a tubular reactor packed with controlled pore glass beads containing immobilized glucose oxidase. The hydr

On-line monitoring of glucose in mammali

On-line monitoring of glucose in mammalian cell culture using a flow injection analysis (FIA) mediated biosensor

✍ Keith B. Male; P. Octavian Gartu; A. Amine Kamen; John H. T. Luong 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 200 KB 👁 2 views

A flow injection analysis (FIA) biosensor system has been developed for on-line determination of glucose during mammalian cell cultivation. The culture sample was peristaltically withdrawn from the bioreactor and after cell separation by a steam sterilizable ceramic microfilter, the filtrate was con