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Development of a lymphocyte transformation assay for rotavirus in whole blood and breast milk

✍ Scribed by B. M. Totterdell; S. Patel; J. E. Banatvala; I. L. Chrystie

Publisher
John Wiley and Sons
Year
1988
Tongue
English
Weight
606 KB
Volume
25
Category
Article
ISSN
0146-6615

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✦ Synopsis

A multivariate assay was developed for detecting lymphoproliferative responses to rotavirus in whole blood and breast milk. Detection of lymphocyte transformation in samples from healthy uninfected adults required an assay incorporating a large number of antigen and cell concentrations and several incubation periods before pulsing. In contrast, lymphoproliferation to rotavirus was observed over a wider range of these variables in blood collected from a kidney recipient nine days post-rotavirus infection. Lyrnphoproliferation to rotavirus was detected in 32.5% of breast milk samples tested. The magnitude of lymphoproliferation to rotavirus did not correlate with rotavirus-specific IgC ELISA antibody titres in sera or with rotavirus-specific IgA ELISA antibody titres in breast milk.

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