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Development of a highly sensitive chemiluminescent assay for hydrogen peroxide under neutral conditions using acridinium ester and its application to an enzyme immunoassay

✍ Scribed by Arakawa, Hidetoshi; Tsuruoka, Keiko; Ohno, Ken-ichi; Tajima, Noriko; Nagano, Hiromi

Book ID: 120547378
Publisher: John Wiley and Sons
Year: 2013
Tongue: English
Weight: 171 KB
Volume: 29
Category: Article
ISSN: 1522-7235
DOI: 10.1002/bio.2555

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The chemiluminescent reaction of lucigenin w i t h various reducing sugars and reducing compounds has been studied. It was found that dihydroxyacetone gave the most intense chemiluminescence (CL). We have developed highly sensitive chemiluminescent methods for alkaline phosphatase (ALP) based on the