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Development of a high-performance thin-layer chromatographic method for the multi-screening analysis of corticosteroids

✍ Scribed by K.E. Vanoosthuyze; L.S.G. Van Poucke; A.C.A. Deloof; C.H. Van Peteghem

Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
384 KB
Volume
275
Category
Article
ISSN
0003-2670

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✦ Synopsis

In order to screen mjectlon sites for the presence of cortlcosterotds, a lugh-pcrfortnance thm-layer chromatographic (HFTLC) system was developed for the determmatlon of 26 cortioostcroid standards, Dtiercnt mobde phases were tested and chromatography ~rlth chloroform-methanol was thoroughly evaluated The development on prcloadcd &es&cl 60 HPTLC plates urlth chloroform-methanol (92 + 8, v/v) welded the best scparatmn After cxamimng dlffcrcnt dctetion methods, a resorcylaldehydc spray was selected, ylcldmg the best colour dflcrcntlatlon and fluorescence at 366 nm for several components Kcywordr Thm-layer chromatography, Cortxostcrolds, Meat, Stcnxds

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