Oligosaccharides released enzymatically by N-glycanase from fetuin, alpha-acid glycoprotein, human chorionic gonadotropin, platelet-derived growth factor, and kallikrein were chromatographed on a polymeric pellicular anion-exchange column at pH values of 5 and 13. Separations occurred into groups of
Development of a high performance anion exchange chromatography analysis for mapping of oligosaccharides
โ Scribed by Carl Grey; Per Edebrink; Maria Krook; Sven P. Jacobsson
- Book ID
- 108161019
- Publisher
- Elsevier Science
- Year
- 2009
- Tongue
- English
- Weight
- 451 KB
- Volume
- 877
- Category
- Article
- ISSN
- 1873-376X
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A simple one-step method for the analysis of monosaccharides including galactosaminitol after acidic hydrolysis is described. The hydrolyzate was re- \(\mathrm{N}\)-acetylated and analyzed by high-performance anion-exchange chromatography and the sugars were detected by a pulsed amperometric detecto
The technique of high-pH anion-exchange chromatography with pulsed amperometric detection has recently been shown to be a powerful method for resolving closely related oligosaccharides [M. R. Hardy and R. R. Townsend, Proc. N&l. Acad. Sci. U.S. A., 85 (1988) 3289932931. This report describes separat