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Development of a High-Affinity Ligand That Binds Irreversibly to Mel 1b Melatonin Receptors

✍ Scribed by Witt-Enderby, Paula A.; Chu, Guo-Hua; Gillen, Megan L.; Li, Pui-Kai


Book ID
127320898
Publisher
American Chemical Society
Year
1997
Tongue
English
Weight
186 KB
Volume
40
Category
Article
ISSN
0022-2623

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A competition-based method is used for the determination of the thermodynamic parameters for a low-affinity ligand binding reaction by isothermal titration calorimetry. This method is based on the coupling of a high-affinity ligand to the binding of the low-affinity ligand. Results are presented for