Low-Affinity Binding Determined by Titra
β
Yan-Ling Zhang; Zhong-Yin Zhang
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Article
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1998
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Elsevier Science
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English
β 187 KB
A competition-based method is used for the determination of the thermodynamic parameters for a low-affinity ligand binding reaction by isothermal titration calorimetry. This method is based on the coupling of a high-affinity ligand to the binding of the low-affinity ligand. Results are presented for