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Development of a genus specific primer set for detection of Leishmania parasites by polymerase chain reaction

✍ Scribed by Riddhi Bhattacharyya; Krishna Das; Sandeep Sen; Syamal Roy; Hemanta K Majumder


Book ID
109320946
Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
512 KB
Volume
135
Category
Article
ISSN
0378-1097

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The development of a quadriplex PCR method with amplification of HCMV in a single-step procedure using primers taken from four different regions of the viral genome is described. Different concentrations of dNTPs and MgCl 2 were assayed in order to optimize the constitution of the buffer for the mul