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Development of a gene expression vector for Thermus thermophilus based on the promoter of the respiratory nitrate reductase

✍ Scribed by Renata Moreno; Olga Zafra; Felipe Cava; José Berenguer


Book ID
114122030
Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
519 KB
Volume
49
Category
Article
ISSN
0147-619X

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Development of a homologous transformati
✍ Horng, Jason S. ;Chang, Perng-Kuang ;Pestka, James J. ;Linz, John E. 📂 Article 📅 1990 🏛 Springer 🌐 English ⚖ 363 KB

The nitrate reductase structural gene (niaD) and an niaD mutant strain were isolated from Aspergillus parasiticus and used to develop a homologous transformation system. A transformation frequency of 110 to 120 transformants per microgram linear DNA was obtained with the 10.9 kb plasmid pSL82, which