Development of a fully integrated "Sample-in-answer-out" system for automatic genetic analysis

✍ Scribed by Zhuang, Bin

• Publisher: Springer
• Year: 2018
• Tongue: English
• Leaves: 131
• Series: Springer theses
• Category: Library

✦ Synopsis

This thesis reports on the development of a fully integrated and automated microsystem consisting of low-cost, disposable plastic chips for DNA extraction and PCR amplification, combined with a reusable glass capillary array electrophoresis chip, which can be employed in a modular-based format for genetic analysis. In the thesis, DNA extraction is performed by adopting a filter paper-based method, followed by an “in-situ” PCR carried out directly in the same reaction chamber of the chip without elution. PCR products are then co-injected with sizing standards into separation channels for detection using a novel injection electrode. The entire process is automatically carried out by a custom-made compact control and detection instrument. The author thoroughly tests the system?s performance and reliability by conducting rapid genetic screening of mutations on congenital hearing loss and pharmacogenetic typing of multiple warfarin-related single-nucleotide polymorphisms. The successful development and operation of this microsystem establishes the feasibility of rapid “sample-in-answer-out” testing in routine clinical practice. Read more...

Abstract: This thesis reports on the development of a fully integrated and automated microsystem consisting of low-cost, disposable plastic chips for DNA extraction and PCR amplification, combined with a reusable glass capillary array electrophoresis chip, which can be employed in a modular-based format for genetic analysis. In the thesis, DNA extraction is performed by adopting a filter paper-based method, followed by an “in-situ” PCR carried out directly in the same reaction chamber of the chip without elution. PCR products are then co-injected with sizing standards into separation channels for detection using a novel injection electrode. The entire process is automatically carried out by a custom-made compact control and detection instrument. The author thoroughly tests the system?s performance and reliability by conducting rapid genetic screening of mutations on congenital hearing loss and pharmacogenetic typing of multiple warfarin-related single-nucleotide polymorphisms. The successful development and operation of this microsystem establishes the feasibility of rapid “sample-in-answer-out” testing in routine clinical practice

✦ Table of Contents

Front Matter....Pages i-xxiii
Introduction....Pages 1-30
Construction of a General Platform for Capillary Electrophoresis....Pages 31-61
Integrated Module for Automated DNA Extraction and Amplification....Pages 63-87
Fully Integrated Genetic Analysis System....Pages 89-109
Conclusions and Prospects for Future Work....Pages 111-114

✦ Subjects

DNA -- Analysis;Microfluidics;SCIENCE / Life Sciences / Biochemistry;DNA -- analysis