Development of a cloning system forStreptomyces, (Volume 21)
β Scribed by M J Bibb; J M Ward; D A Hopwood
- Book ID
- 110050569
- Publisher
- Springer-Verlag
- Year
- 1999
- Tongue
- English
- Weight
- 737 KB
- Volume
- 22
- Category
- Article
- ISSN
- 1476-5535
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In developing a system for molecular cloning with the Podospora anserina plasmid (plDNA) it is necessary to find recipient strains which are resistant to plDNA mediated senescence. Three long lived double mutants which fail to exhibit spontaneous aging were genetically and biochemically analysed. Al
A plasmid-cloning system was developed for Streptomyces viridochromogenes Tu494, a producer of the tripeptide antibiotic phosphinothricyl-alanyl-alanine (PTT). Parameters affecting protoplast formation and transformability of S. viridochromogenes were investigated in detail. A procedure giving rise