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Development of a bioreactor based on trypsin immobilized on monolithic support for the on-line digestion and identification of proteins

✍ Scribed by E. Calleri; C. Temporini; E. Perani; C. Stella; S. Rudaz; D. Lubda; G. Mellerio; J.-L. Veuthey; G. Caccialanza; G. Massolini

Book ID
108092175
Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
175 KB
Volume
1045
Category
Article
ISSN
1873-3778

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## Abstract An integrated platform consisting of protein separation by CIEF with monolithic immobilized pH gradient (M‐IPG), on‐line digestion by trypsin‐based immobilized enzyme microreactor (trypsin‐IMER), and peptide separation by CZE was established. In such a platform, a tee unit was used not