Development of a baculovirus expression system for soluble porcine tumor necrosis factor receptor type I and soluble porcine tumor necrosis factor receptor type I-IgG fusion protein
β Scribed by Simon Musyoka Mwangi; Thomas J. Stabel; Marcus E. Kehrli Jr.
- Book ID
- 117335935
- Publisher
- Elsevier Science
- Year
- 2002
- Tongue
- English
- Weight
- 104 KB
- Volume
- 86
- Category
- Article
- ISSN
- 0165-2427
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
We evaluated the effect of UV-B light at 302 nm on a model therapeutic protein, 2.6 D type I soluble tumor necrosis factor receptor (sTNF-RI). This protein contains a single Trp at position 97 and seven native disulfide bonds along its interior from the N to the C-terminus. At a protein concentratio
In this study optimization of the soluble tumor necrosis factor receptor type I (sTNF-RI) refolding by the use of a micro-renaturation assay in 96-well microplates is described. Microplate wells were filled with buffers varying in pH and urea and substrate concentration. Denatured and reduced sTNF-R