Development in vitro of the early chick blastoderm explanted on yolk and albumen extract saline-agar substrata
✍ Scribed by Spratt, Nelson T.
- Publisher
- John Wiley and Sons
- Year
- 1947
- Tongue
- English
- Weight
- 1001 KB
- Volume
- 106
- Category
- Article
- ISSN
- 0022-104X
No coin nor oath required. For personal study only.
✦ Synopsis
It is somewhat surprising, in view of its obvious usefulness, that the technique of tissue culture, introduced to biologists by Dr. Ross Harrison in 1907, has not been employed in the study of the general problem of early embryonic nutrition. Although many studies have been made of the nutritive requirements of tissue cells isolated from relatively old chick embryos (4-8 days) and explanted in vitro, none have been made of the intact blastoderrn during the first 3 days of its development on various media in vitro. Some of the pertinent questions which one might ask are the following: Does the early blastoderm depend upon some exogenous source of energy for its early development, or does