Neuronal and glial cells in primary culture prepared from the hypothalamic-brain stem areas of one-day-old rat brains were analyzed for the presence of angiotensinogen messenger RNA (mRNA) to further confirm our previous conclusion that the brain contains an exclusive angiotensin system. Angiotensin
Development expression of glial-specific mRNAs in primary cultures of rat brain visualized by in situ hybridization
β Scribed by E. Holmes; G. Hermanson; R. Cole; Dr. J. de Vellis
- Publisher
- John Wiley and Sons
- Year
- 1988
- Tongue
- English
- Weight
- 1010 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0360-4012
No coin nor oath required. For personal study only.
β¦ Synopsis
The localization of mRNAs which encode the glial-uninduced mixed glial cultures. In future studies, the specific marker proteins, glial fibrillary acidic protein hormonal regulation of glial-specific mRNAs will be (GFAP), glycerol phosphate dehydrogenase (GPDH, compared to the basal gene activation presented here. EC 1.1.1.8), and myelin basic protein (MBP), was mapped by in situ hybridization in cultures Key words: astrocytes, oligodendrocytes, glial fibrildium. Developmental changes of these expressed nase, mRNAs were examined after various times in culture
π SIMILAR VOLUMES
Monoamine oxidases A and B (MAOA and MAOB) are the major catabolic isoenzymes of catecholamines and serotonin in the mammalian brain. Although the distribution of the monoamine oxidase protein has been mapped by ligand binding and immunohistochemistry, the sites of MAOA and MAOB synthesis have not b
Although accumulating data reveal patterns of proliferation, migration, and differentiation of neuronal lineage cells in the developing brain, gliogenesis in the brain has not been well elucidated. In the rat brain, vimentin is selectively expressed in radial glia and in their progeny, not in oligod
The present work was undertaken to study the effect of bacterial lipopolysaccharide (LPS), a potent activator of the host inflammatory response, on the synthesis of nerve growth factor (NGF) by newborn rat brain astrocytes. Treatment of primary rat astroglial cells cultured in chemically defined med
Increases in the mRNA levels of two neuroglial markers, glutamine synthetase (EC 6.3.1.2; GS) and glycerolphosphate dehydrogenase (EC 1.1.1.8; GPDH), were observed in hydrocortisone-treated cultures of astrocytes and oligodendrocytes, respectively, and in C6 cells by Northern blot analysis and in si