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Development and viability of one- and two-cell porcine embryos cultured through the “four-cell block”

✍ Scribed by L.S. Beckmann; T.C. Cantley; A.R. Rieke; B.N. Day

Book ID
118378687
Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
78 KB
Volume
33
Category
Article
ISSN
0093-691X

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The effect of increasing the embryo:medium volume ratio on overcoming the hamster two-cell block was examined. Two-cell golden hamster embryos from each superovulated female were cultured in microdrops (estimated at 0.75 microliter) or 100 microliter macrodrops of chemically defined medium (modified