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Development and Validation of Oxygen Radical Absorbance Capacity Assay for Lipophilic Antioxidants Using Randomly Methylated β-Cyclodextrin as the Solubility Enhancer

✍ Scribed by Huang, Dejian; Ou, Boxin; Hampsch-Woodill, Maureen; Flanagan, Judith A.; Deemer, Elizabeth K.

Book ID: 111987705
Publisher: American Chemical Society
Year: 2002
Tongue: English
Weight: 91 KB
Volume: 50
Category: Article
ISSN: 0021-8561
DOI: 10.1021/jf0113732

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An improved method of oxygen radical absorbance capacity (ORAC) assay has been developed and validated using fluorescein (3′,6′-dihydroxyspiro[isobenzofuran-1[3H],9′[9H]-xanthen]-3-one) as the fluorescent probe. Our results demonstrate that fluorescein (FL) is superior to B-phycoerythrin. The oxidiz