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Development and validation of a quantitative ELISA for the measurement of PSA concentration

✍ Scribed by Boris Acevedo; Yasser Perera; Miriam Ruiz; Gertrudis Rojas; Jesús Benı́tez; Marta Ayala; Jorge Gavilondo

Book ID
117074519
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
150 KB
Volume
317
Category
Article
ISSN
0009-8981

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✍ Dr. James T. Wu; Lorraine W. Wilson 📂 Article 📅 1995 🏛 John Wiley and Sons 🌐 English ⚖ 772 KB

An ELISA on microplate was established for the total serum PSA. We selected the monoclonal antibody for the assay from commercial sources making certain that it reacted with both free PSA and PSA-a,-antichymotrypsin (PSA-ACT) complex from human serum with similar affinity (so-called "equimolar"). We