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Development and Validation of a Gamma Interferon ELISPOT Assay for Quantitation of Cellular Immune Responses to Varicella-Zoster Virus

✍ Scribed by Smith, J. G.; Liu, X.; Kaufhold, R. M.; Clair, J.; Caulfield, M. J.


Book ID
121789463
Publisher
American Society for Microbiology
Year
2001
Tongue
English
Weight
219 KB
Volume
8
Category
Article
ISSN
1556-6811

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An interferon-gamma ELISPOT assay has been developed for assessment of cellular immune responses to Varicella-Zoster Virus (VZV) in large, multi-center clinical vaccine trials. We show that the assay performed best when testing peripheral blood mononuclear cells (PBMCs) that had been isolated and th