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Development and evaluation of human AP endonuclease inhibitors in melanoma and glioma cell lines

✍ Scribed by Mohammed, M Z; Vyjayanti, V N; Laughton, C A; Dekker, L V; Fischer, P M; Wilson, D M; Abbotts, R; Shah, S; Patel, P M; Hickson, I D


Book ID
110000834
Publisher
Nature Publishing Group
Year
2011
Tongue
English
Weight
820 KB
Volume
104
Category
Article
ISSN
0007-0920

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The human AP endonuclease (APE) activity counteracts the mutagenic and cytotoxic effects of the frequent genomic lesions abasic (AP) sites. In order to investigate the interindividual variability of APE levels, a simple and quantitative assay was developed. Crude lymphocyte extracts were incubated w