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Development and Evaluation of EPA Method 1615 for Detection of Enterovirus and Norovirus in Water

✍ Scribed by Cashdollar, J. L.; Brinkman, N. E.; Griffin, S. M.; McMinn, B. R.; Rhodes, E. R.; Varughese, E. A.; Grimm, A. C.; Parshionikar, S. U.; Wymer, L.; Fout, G. S.

Book ID
118280098
Publisher
American Society for Microbiology
Year
2012
Tongue
English
Weight
409 KB
Volume
79
Category
Article
ISSN
0099-2240

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## Abstract A two‐step reverse transcriptase TaqMan™ duplex PCR (RT‐PCR) assay was developed using the ABI 7700 Sequence Detection System for the detection of enterovirus (EV) and parechovirus type 1 and 2 (PEV) RNA from samples of cerebrospinal fluid (CSF) and throat swabs. Using sequence‐specific