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Development and evaluation of an antibody capture ELISA for detection of IgG to Epstein-Barr virus in oral fluid samples

✍ Scribed by Carmen Sheppard; Bernard Cohen; Nick Andrews; Heidi Surridge


Book ID
114139637
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
135 KB
Volume
93
Category
Article
ISSN
0166-0934

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A 3-step enzyme-linked immunosorbent assay (ELISA) was developed for detecting IgA antibodies to purified Epstein-Barr virus (EBV) polypeptides. The 3-step procedure included the use of a mouse anti-human IgA monoclonal antibody (MAb) to amplify the IgA reaction. The 2 major EBV proteins used in thi