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Development and Evaluation of a PCR Based Assay for Detection of the Toxic Dinoflagellate,Gymnodinium catenatum(Graham) in Ballast Water and Environmental Samples

✍ Scribed by Jawahar G. Patil; Rasanthi M. Gunasekera; Bruce E. Deagle; Nicholas J. Bax; Susan I. Blackburn


Publisher
Springer
Year
2005
Tongue
English
Weight
285 KB
Volume
7
Category
Article
ISSN
1387-3547

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## Abstract A two‐step reverse transcriptase TaqManβ„’ duplex PCR (RT‐PCR) assay was developed using the ABI 7700 Sequence Detection System for the detection of enterovirus (EV) and parechovirus type 1 and 2 (PEV) RNA from samples of cerebrospinal fluid (CSF) and throat swabs. Using sequence‐specific