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Development and assessment of a novel real-time PCR assay for quantitation of hepatitis D virus RNA to study viral kinetics in chronic hepatitis D

✍ Scribed by A. Katsoulidou; E. Manesis; C. Rokka; C. Issaris; A. Pagoni; V. Sypsa; A. Hatzakis

Book ID
119878455
Publisher
John Wiley and Sons
Year
2012
Tongue
English
Weight
260 KB
Volume
20
Category
Article
ISSN
1352-0504

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✍ Olmedo, Eva; Costa, Josep; LοΏ½pez-Labrador, Fancesc X.; Forns, Xavier; AmpurdanοΏ½s πŸ“‚ Article πŸ“… 1999 πŸ› John Wiley and Sons 🌐 English βš– 169 KB πŸ‘ 2 views

A modified competitive RT-PCR (mcRT-PCR) to measure HCV RNA in serum and the Amplicor HCV Monitor assay were compared. For mcRT-PCR, the RNA extracted was retrotranscribed and coamplified in one step with a known amount of a DNA internal control (IC). Digoxigenin-labeled amplified products were hybr