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Development and analytical validation of a novel assay for detection of human Aβ1-42 peptide in human CSF

✍ Scribed by Flora Berisha; Jill Dunty; Leonid Dzantiev; Adam Simon; Carol Gleason; Oitak (Allen) Wong; Mwanatumu Mbwana; Sara Hapip; Qian Ning; Franklin Braffett; Sarah Robles; George Green; Robert Neely; Holly Soares; James Wilbur; Pankaj Oberoi; Paul Rhyne; David Stewart


Book ID
118468662
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
171 KB
Volume
8
Category
Article
ISSN
1552-5260

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3-Endorphin-like peptides in blood plasma of normal human subjects were studied by means of a radioimmunoassay (RIA) and gel filtration. Plasma was extracted with silica gel, which was washed with water and 1 N HCI, and eluted with 50% acetone. Plasma extracts thus obtained and standard synthetic hu