𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Deuterium isotope effects on β-galactosidase formation by Escherichia coli

✍ Scribed by T.R. Henderson; Judy M. Dacus; H.L. Crespi; J.J. Katz


Book ID
118845617
Publisher
Elsevier Science
Year
1967
Tongue
English
Weight
506 KB
Volume
120
Category
Article
ISSN
0003-9861

No coin nor oath required. For personal study only.


📜 SIMILAR VOLUMES


Solvent Deuterium Isotope Effect on the
✍ John P. Richard; Deborah A. McCall 📂 Article 📅 2000 🏛 Elsevier Science 🌐 English ⚖ 68 KB

A value of 1.8 has been determined for (K I ) HOH /(K I ) DOD , the ratio of the values of K I for competitive inhibition of β-galactosidase by isopropyl β-D-thiogalactopyranoside in H 2 O and D 2 O. This is similar to the value of 1.7 for (K m ) HOH /(K m ) DOD , the ratio of the Michaelis constant

Effect of an E461G Mutation of β-Galacto
✍ John P. Richard; Reuben E. Huber; Deborah A. McCall 📂 Article 📅 2001 🏛 Elsevier Science 🌐 English ⚖ 116 KB

An E461G mutation of ␤-galactosidase results in the disappearance of the high pL (L ϭ H, D) downward break in the rate profiles for k cat /K m for wild-type enzyme-catalyzed hydrolysis of 4-nitrophenyl ␤-D-galactopyranoside (Gal-OPNP) and a decrease from (k cat ) HOH /(k cat ) DOD ϭ 1.7 to (k cat )