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Deuterium isotope effects in nonenzymatic transamination of L-glutamic acid

✍ Scribed by Song-Ling Ljn; Martin I. Blake; Frederick P. Siegel

Publisher
John Wiley and Sons
Year
1965
Tongue
English
Weight
579 KB
Volume
54
Category
Article
ISSN
0022-3549

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✦ Synopsis

L-Deuterio-glutamic acid was isolated in pure form from algae grown in nutrient solution containing better than 99 per cent Dz0. The pH profile for the transamination of glutamic acid with pyridoxal shows a peak at pH 4. The presence of acetate buffer increases the reaction rate up to about seven times when com ared to the unbuffered system. Protio-glutamic acid reacts about 2.1 times faster tian deuterio-glutamic acid. The reaction appears to be general acid and general base catalyzed.

N BIOLOGICAL systems transamination involves I the transfer of an amino group between certain amino and keto acids. The discovery of the occurrence in nature of the aldehyde and amine forms of vitamin Ba by Snell(1) led to the suggestion that pyridoxal and pyridoxamine may be interconvertible in transamination reactions, and

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