Determining the mechanical properties of yeast cell walls
β Scribed by John D. Stenson; Peter Hartley; Changxiang Wang; Colin R. Thomas
- Publisher
- American Institute of Chemical Engineers
- Year
- 2011
- Tongue
- English
- Weight
- 208 KB
- Volume
- 27
- Category
- Article
- ISSN
- 8756-7938
- DOI
- 10.1002/btpr.554
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
The rate of formation of spheroplasts of yeast can be used as an assay to study the structural integrity of cell walls. Lysis can be measured spectrophotometrically in hypotonic solution in the presence of Zymolyase, a mixture of cell wall-digesting enzymes. The optical density of the cell suspensio
A reliable acid hydrolysis method for quantitative determination of the proportion of -glucan, mannan and chitin in Saccharomyces cerevisiae cell wall is reported together with a simple extraction procedure to quantify within a standard error of less than 2% the proportion of the wall per gram of ce
The e β ect of temperature and moisture on the fabrication of pressed carrot cell wall specimens for Dynamic Mechanical Thermal Analysis was assessed. Results obtained from the water extractability of the material showed that more cell wall material became solubilised when moisture and temperature of