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Determining molecular weight distributions of antigen–antibody complex by quasi-elastic light scattering

✍ Scribed by Regina M. Murphy; Martin L. Yarmush; Clark K. Colton


Publisher
Wiley (John Wiley & Sons)
Year
1991
Tongue
English
Weight
576 KB
Volume
31
Category
Article
ISSN
0006-3525

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✦ Synopsis


All molecular weights are in thousands. Methods: (A) EM. (B) Thermodynamic model. ( C ) Size distributions from method B analyzed by CONTIN. (D) QLS data analyzed by CONTIN. (E) CLS.


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We report the first characterization of group B meningococcal polysaccharide a t 28OC in 0.4M KC1 and 0.05M sodium acetate at pH 5.61 and in pure water by means of light-scattering spectroscopy. In buffer solution, the weight-average molecular weight M, = 1.46 X lo5 g/mol, the 2-average radius of gy