Determining acrosomal status of the cynomolgus monkey (Macaca fascicularis) sperm by fluorescence microscopy

✍ Scribed by Dr. Nicholas L. Cross; Patricio Morales; Masaru Fukuda; Esmail Behboodi

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 453 KB
• Volume: 17
• Category: Article
• ISSN: 0275-2565
• DOI: 10.1002/ajp.1350170205

✦ Synopsis

The acrosome of Macaca fascicularis sperm cannot be distinguished by conventional light microscopy, so determining whether sperm are acrosome-intact or -reacted is difficult. We describe methods for labeling the acrosomal region of sperm with two different probes: f luoresceinated Pisum sativum agglutinin and anti-sperm antiserum. Acrosome-intact sperm are much more heavily labeled in the acrosomal region than are acrosomereacted sperm, providing a simple means of differentiating the two types of sperm. The two probes detect similar numbers of acrosome-reacted sperm following treatment with the divalent cation ionophore, A23187.