Root segments of Pisum sativum L. were transformed by several strains of Agrobacterium rhizogenes. The resulting hairy roots, as well as apical segments from untransformed pea roots, were used to initiate root lines cultured in vitro. Levels of free IAA were quantified in the sub-cultured lines by g
✦ LIBER ✦
Determinations of indole-3-acetic acid in xylem sap ofRicinus communisL. using mass fragmentography
✍ Scribed by J. R. F. Allen; A. M. Greenway; D. A. Baker
- Publisher
- Springer-Verlag
- Year
- 1979
- Tongue
- English
- Weight
- 370 KB
- Volume
- 144
- Category
- Article
- ISSN
- 0032-0935
No coin nor oath required. For personal study only.
✦ Synopsis
Mass fragmentography employing a deuterated internal standard was used to make quantitative analyses of indole-3-acetic acid in xylem sap collected from Ricinus communis L. When contamination of the sap by microorganisms was reduced by frequent collection, levels of IAA were found to be less than 0.5 ng ml(-1). It is therefore proposed that the transpiration stream does not play a significant role in the transport of IAA within the plant.
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